Tryptophan Hydroxylase 1 Antikörper

Produktnummer ABIN372730

Dieser Schaf Polyklonal Antikörper detektiert spezifisch Tryptophan Hydroxylase 1 in WB und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für Tryptophan Hydroxylase 1 Antikörper (ABIN372730)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reaktivität: Human, Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Spezifität: Specific for the ~55k Tryptophan Hydroxylase protein. The antibody has been directly tested for reactivity in Western blots with Human and Rat tissue. Based upon the relatively high degree of homology of Tryptophan Hydroxylase, the antibodies should cross-react with other mammalian species. Does not recognize TPH in Rabbit tissues.

Kreuzreaktivität (Details): Species reactivity (tested):Human and Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Recombinant rabbit Tryptophan Hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1/1,000. Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: Tryptophan 5-Hydroxylase 1 (TPH1)

Hintergrund: Tryptophan Hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1

Gen-ID: 7166

NCBI Accession: NP_004170

UniProt: P17752

Pathways: Feeding Behaviour