IL1R1 Antikörper (Extracellular Domain)

Produktnummer ABIN364253

Der Maus Monoklonal anti-IL1R1 Antikörper wird verwendet zum Nachweis von IL1R1 in Proben von Human. Er wurde validiert für IHC, ELISA, IHC (p) und RIA.

Kurzübersicht für IL1R1 Antikörper (Extracellular Domain) (ABIN364253)

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL1R1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Klon: 40101

Produktdetails anti-IL1R1 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: These antibodies react with cell surface and soluble human IL-1 receptor, Specific epitopes are unknown, but they do partially inhibit (30-50 % ) binding of one another to IL-1 receptor. They do not block binding of IL-1 to receptor, and they do not cross-react with receptors for human IL-2 or IL-4.

Aufreinigung: Protein G purified

Immunogen: The extracellular domain of the type I human IL-1 receptor.

Type of Immunogen: Protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (10 μg/mL), RIA

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA: Can be used as capture reagents or second antibody at a concentration of 5 μg/mL. RIA: Concentrations of 20-30 ng/mL of these antibodies will bind radiolabeled IL-1 receptor in a radioimmunoassay format. Immunocytochemistry: IL-1 receptor can be detected on cultured cells using antibody concentrations of 10-100 μg/mL.

Not recommended for: WB

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4. Sourced in Ascites.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Short term: -20°C
Long term: -70°C
Avoid freeze-thaw cycles.

Details zu IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Andere Bezeichnung: IL1RA / IL1R1

Hintergrund: Name/Gene ID: IL1R1
Family: Interleukin

Synonyms: IL1R1, Antigen CD121a, D2S1473, IL-1R-1, IL-1R-alpha, IL-1RT-1, IL-1RT1, IL1R, IL1RA, IL1RT1, Interleukin receptor 1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, CD121A, CD121a antigen, Interleukin 1 receptor, type I, Interleukin-1 receptor type I, Interleukin-1 type i receptor, p80

Gen-ID: 3554

UniProt: P14778

Pathways: NF-kappaB Signalweg, Carbohydrate Homeostasis, Cancer Immune Checkpoints