ASK1 Antikörper (pSer966)
Kurzübersicht für ASK1 Antikörper (pSer966) (ABIN362195)
Target
Alle ASK1 (MAP3K5) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- pSer966
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Spezifität
- The antibody detects endogenous level of ASK1 only when phosphorylated at serine 966.
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Aufreinigung
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogen
- Peptide sequence around phosphorylation site of pSer966 (S-I-S (p) -L-P) derived from Human ASK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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Isotyp
- IgG
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Applikationshinweise
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells." in: Journal of cellular biochemistry, Vol. 110, Issue 3, pp. 581-8, (2010) (PubMed).
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- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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Andere Bezeichnung
- ASK1
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Hintergrund
- Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively.
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Molekulargewicht
- 155 kDa
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NCBI Accession
- NP_005914
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UniProt
- Q99683
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Pathways
- MAPK Signalweg, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
Target
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