Retinoblastoma 1 Antikörper (pSer795)

Produktnummer ABIN362113

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Retinoblastoma 1 in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für Retinoblastoma 1 Antikörper (pSer795) (ABIN362113)

Target: Retinoblastoma 1 (RB1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Retinoblastoma 1 Antikörper

Bindungsspezifität: pSer795

Spezifität: The antibody detects endogenous level of Rb only when phosphorylated at serine 795.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer795 (P-S-S (p) -P-L) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-Retinoblastoma 1 Antikörper (ABIN362113)

Braumüller, Wieder, Brenner, Aßmann, Hahn, Alkhaled, Schilbach, Essmann, Kneilling, Griessinger, Ranta, Ullrich, Mocikat, Braungart, Mehra, Fehrenbacher, Berdel, Niessner, Meier, van den Broek et al.: "T-helper-1-cell cytokines drive cancer into senescence. ..." in: Nature, Vol. 494, Issue 7437, pp. 361-5, (2013) (PubMed).

Sun, Fu, Liu, Tie, Zhu, Xing, Sun, Zheng: "Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest." in: FEBS letters, Vol. 582, Issue 10, pp. 1564-8, (2008) (PubMed).

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: Rb

Hintergrund: Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Molekulargewicht: 110 kDa

NCBI Accession: NP_000312

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie