Nitrotyrosine Antikörper

Antigen: Nitrotyrosine

Klonalität: Monoklonal (39B6)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Hund

Applikation: Western Blot (WB), ELISA, Immunhistochemie (IHC), Immunpräzipitation (IP)

Produktnummer: ABIN361658

Produktbeschreibung

Produktmerkmale: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Immunogen: Hybridoma line 39B6

Format: Liquid

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 39B6

Beschreibung: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling . While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury. The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.

Spezifität: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific. Species cross-reactivity: Human, Mouse, Rat, Dog.

Sensitivität: 0.7µg/ml of SMC-154 was sufficient for detection of 5µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Anwendungen

Konzentration: 1 mg/mL

Reinigung: Protein G purified

Buffer: PBS, 50% glycerol

Lagerung: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Forschungsgebiet: Metabolismus, Aminosäuren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Nitrotyrosine (39B6), mouse backskin

Nitrotyrosine (39B6), rat liver