Superoxide Dismutase 2, Mitochondrial (SOD2) Antikörper

Antigen: Superoxide Dismutase 2, Mitochondrial (SOD2)

Synonyme: MnSOD, Sod-2, MGC6144, LOC100101896, Sod2, MNSOD, IPO-B, MVCD6, Mn-SOD, MGC128371, cb463, zgc:73051, wu:fj33b01, GB14346, sod2, MGC88869, SOD2, DKFZp459E042, LOC100282741

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Meerschweinchen, Maus, Rind (Kuh), Hund, Huhn, Fruchtfliege (Drosophila melanogaster), Schwein, Hamster, Affe, Kaninchen, Schaf, Xenopus laevis

Applikation: Western Blot (WB), Immunpräzipitation (IP), ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN361656

Produktbeschreibung

Produktmerkmale: Detects a ~25kDa protein corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS PAGE immunoblots.

Weitere Bezeichnung: Mn SOD

Gen-ID: NP_058747.1, 24787

Immunogen: Rat Mn SOD

Format: Liquid

Beschreibung: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Synonyms: Manganese SOD, IPO B, Mn SOD, SOD2

Spezifität: Detects 25kDa protein, corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS-PAGE immunoblots. Species cross-reactivity: Human, Rat, Mouse, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus.

Anwendungen

Applikationshinweise: 0.5ug/ml was sufficient for detection of Mn SOD in 20ug of rat brain tissue extract

Konzentration: 1mg/mL

Buffer: PBS pH7, 50% glycerol, 0.09% sodium azide

Lagerung: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Forschungsgebiet: Oxidativer Stress

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Mn SOD (Rat), mouse backskin