TNFRSF18 Antikörper

Produktnummer ABIN360890

Der Ratte Monoklonal anti-TNFRSF18 Antikörper wird verwendet zum Nachweis von TNFRSF18 in Proben von Maus. Er wurde validiert für FACS und IHC (p).

Kurzübersicht für TNFRSF18 Antikörper (ABIN360890)

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TNFRSF18 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 7C13

Produktdetails anti-TNFRSF18 Antikörper

Spezifität: This antibody recognizes TNFRSF18/GITR.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Protein A/G Affinity Chromatography

Immunogen: Mouse myeloma fused with spleen cells from a Rat immunized with Mouse T cells.

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: FACS Analysis: 1/50-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 200 μL sterile PBS (final concentration 500 μg/mL).

Buffer: PBS without preservatives or stabilizers

Konservierungsmittel: Without preservative

Vorsichtsmaßnahmen: Do Not Use Sodium Azide as preservative.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Details zu TNFRSF18

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Andere Bezeichnung: TNFRSF18 / GITR

Hintergrund: TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells.Synonyms: AITR, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein, Tumor necrosis factor receptor superfamily member 18

Gen-ID: 8784

NCBI Accession: NP_004186

UniProt: Q9Y5U5

Pathways: Cancer Immune Checkpoints