Epidermal Growth Factor Receptor (EGFR) (pTyr992) Antikörper

Antigen: Epidermal Growth Factor Receptor (EGFR)

Synonyme: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Epitope: pTyr992

Klonalität: Monoklonal (EM-12)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN343716

Produktbeschreibung

Weitere Bezeichnung: EGFR (Phospho-Tyr992)

Immunogen: Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: EM-12

Beschreibung: The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, isa member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDatransmembrane glycoprotein is often associated with cancerogenesis, although its activationstate is controlled at various levels including trafficking and degradation steps. Binding ofreceptor-specific ligands to the EGFR ectodomain results in formation of homodimeric orheterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferredpartner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways leadto cell proliferation and survival responses.

Spezifität: The antibody EM-12 reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine992.

Synonyme: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: EGF-stimulated A431. Negative control: Non-stimulated A431

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).