TRIM5 Antikörper (AA 2-15)

Produktnummer ABIN337284

Der Ziege Polyklonal anti-TRIM5 Antikörper (ABIN337284) detektiert spezifisch TRIM5 in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für TRIM5 Antikörper (AA 2-15) (ABIN337284)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRIM5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TRIM5 Antikörper

Bindungsspezifität: AA 2-15

Spezifität: Human TRIM5. This antibody is expected to recognise all three human isoforms of this protein.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%) Tamarin, Horse (93%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide ASGILVNVKEEVTC from the N-terminus of human TRIM5 (NP_149023.1, NP_149083.1, NP_149084.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%), Tamarin, Horse (93%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Andere Bezeichnung: TRIM5

Hintergrund: Name/Gene ID: TRIM5
Family: Tripartite Motif

Synonyms: TRIM5, RNF88, RING finger protein 88, Tripartite motif containing 5, TRIM5alpha, Tripartite motif protein TRIM5, Tripartite motif-containing 5

Gen-ID: 85363

NCBI Accession: NP_149023, NP_149083, NP_149084

Pathways: Activation of Innate immune Response