CK1 epsilon Antikörper (AA 403-416)

Produktnummer ABIN337139

Der Ziege Polyklonal anti-CK1 epsilon Antikörper wird verwendet zum Nachweis von CK1 epsilon in Proben von Human. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für CK1 epsilon Antikörper (AA 403-416) (ABIN337139)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CK1 epsilon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CK1 epsilon Antikörper

Bindungsspezifität: AA 403-416

Spezifität: Human CSNK1E. Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Turkey, Chicken, Platypus (93%) Xenopus (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-PASQTSVPFDHLGK from the C-terminus of human CSNK1E (NP_001885.1, NP_689407.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Turkey, Chicken, Platypus (93%), Xenopus (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Andere Bezeichnung: CSNK1E / CK1 Epsilon

Hintergrund: Name/Gene ID: CSNK1E
Subfamily: Casein kinase I
Family: Protein Kinase

Synonyms: CSNK1E, Casein kinase 1 epsilon, Casein kinase i epsilon, CK1 epsilon, CKIE, CKIepsilon, CKI-epsilon, HCKIE, Casein kinase 1, epsilon

Gen-ID: 1454

NCBI Accession: NP_001885, NP_689407

UniProt: P49674

Pathways: Hedgehog Signalweg, M Phase