P4HB Antikörper

Produktnummer ABIN337100

Der Kaninchen Polyklonal anti-P4HB Antikörper wird verwendet zum Nachweis von P4HB in Proben von Human. Er wurde validiert für WB, IHC, ICC, IF und IHC (p).

Kurzübersicht für P4HB Antikörper (ABIN337100)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P4HB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-P4HB Antikörper

Spezifität: Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the sequence around Pro329 of human PDI (KLH).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:50), IF (1:50), IHC, IHC-P (1:50), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as a liquid in 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: PDI / P4HB

Hintergrund: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

Gen-ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism