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Heat Shock 27kDa Protein 1 (HSPB1) Antikörper

Antigen

Heat Shock 27kDa Protein 1 (HSPB1)

Synonyme
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, M ... show more
Klonalität Monoklonal (2A5)
Wirt
Reaktivität
Alternativen

Human

Applikation
Alternativen Enzyme Immunoassay (EIA), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Produktnummer ABIN317528
Menge 0.1 ml  (1.0 mg/ml)  (Varianten)
Preis 310,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Versandbereit innerhalb von 7 bis 10 Werktagen

Produktbeschreibung

Produktmerkmale Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
Weitere Bezeichnung HSPB1 / HSP27
Swiss-Prot P04792
Immunogen Recombinant human Hsp27 (1-205 aa) purified from E. coli
Isotyp IgG1  (Passende Sekundärantikörper)
Klon 2A5
Beschreibung Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of themammalian small heat shock protein family. Hsp27 is expressed constitutively in manytissues and its expression is increased to high levels after various types of stress includingelevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated invivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases includingMAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Hsp27 forms amultimeric complex within cells, and its primary chaperone function, like other small Hsps,appears to be the stabilization of denatured or aggregated proteins in a refoldingcompetent state.
Spezifität The antibody recognizes human Hsp27. Other species not tested.
Synonyme CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259

Anwendungen

Applikationshinweise ELISA. Western blot (1: 1,000 - 1: 2,000). Immunohistochemistry on Parffin embedded tissue (1: 50). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Konzentration 1.0 mg/ml
Buffer PBS, pH 7.4, containing 0.09% sodium azide
Lagerung Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "Heat Shock 27kDa Protein 1 (HSPB1)", Typ "Antikörper" finden
Wirte Kaninchen (148), Maus (84), Huhn (2)
Reaktivitäten Human (217), Ratte (Rattus) (48), Maus (45), Rind (Kuh) (14), Hund (14), Affe (14), Huhn (10), Meerschweinchen (9), Schwein (8), Hamster (7), Säugetiere (6), Fisch (4), Ziege (4), Katze (3), Pferd (2), Makake (2), Schaf (2), Forelle (2), Vogel (1), Nerz (1), Pute (1)
Applikationen Western Blot (WB) (198), Immunhistochemie (Paraffinschnitte) (IHC (p)) (64), Immunhistochemie (IHC) (56), Immunpräzipitation (IP) (53), ELISA (46), Immunfluoreszenz (IF) (44), Enzyme Immunoassay (EIA) (34), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (31), Immunzytochemie (ICC) (18), Durchflusszytometrie (FACS) (9), Dot Blot (Dot) (4), Immunelektronenmikroskopie (EM) (2), ELISA (Detektionsantikörper) (1), Radioimmunoassay (RIA) (1)
Konjugate Biotin (7), FITC (5), DY488 (1)
Epitope pSer82 (18), pSer78 (16), pSer15 (13), p (4), S78 (2), pSer83 (2), Center (1), S15 (1), S82 (1), S83 (1), pS83 (1), pSer254 (1), pSer78/82 (1), pSer85 (1), pSer86 (1), pTyr512 (1)