GLUT1 Antikörper (C-Term)

Produktnummer ABIN307185

Der Kaninchen Polyklonal anti-GLUT1 Antikörper (ABIN307185) detektiert spezifisch GLUT1 in WB, IHC, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für GLUT1 Antikörper (C-Term) (ABIN307185)

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GLUT1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes human Glut-1.

Aufreinigung: Immunoaffinity purified

Immunogen: A synthetic peptide derived from C-terminal of human GLUT-1Cellular Localization: Membrane and focal cytoplasmic.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-P (5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2-4 μg/mL for 2hrs. Positive control: Rat brain lysate, breast carcinoma, squamous cell carcinoma, non-small-cell lung carcinoma, esophageal carcinoma.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.2 % BSA, 15 mM sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GLUT1

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Andere Bezeichnung: SLC2A1 / GLUT-1

Hintergrund: Name/Gene ID: SLC2A1
Subfamily: Sugar transporter
Family: Transporter

Synonyms: SLC2A1, EIG12, GLUT1DS, DYT17, DYT18, DYT9, Glucose transporter 1, HepG2 glucose transporter, Glucose transporter GLUT1, GLUT-1, GLUT1, PED

Gen-ID: 6513

UniProt: P11166

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Warburg Effekt