GFAP Antikörper (AA 417-430)

Produktnummer ABIN305893

Dieser Ziege Polyklonal Antikörper detektiert spezifisch GFAP in WB, IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Affe und Fledermaus.

Kurzübersicht für GFAP Antikörper (AA 417-430) (ABIN305893)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Affe, Fledermaus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GFAP Antikörper

Bindungsspezifität: AA 417-430

Spezifität: Human GFAP.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rat, Bat, Bovine, Elephant, Horse, Rabbit (100%) Mouse, Dog, Pig, Turkey, Chicken (93%) Opossum, Lizard (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DGEVIKESKQEHKD from the C-terminus of human GFAP (NP_002046.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rat, Bat, Bovine, Elephant, Horse, Rabbit (100%), Mouse, Dog, Pig, Turkey, Chicken (93%), Opossum, Lizard (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund: Name/Gene ID: GFAP

Synonyms: GFAP

Gen-ID: 2670

NCBI Accession: NP_002046

UniProt: P14136