GLI3 Antikörper (AA 41-57)

Produktnummer ABIN303380

Der Kaninchen Polyklonal anti-GLI3 Antikörper wird verwendet zum Nachweis von GLI3 in Proben von Human, Ratte, Huhn, Hund, Affe, Wachtel und Frosch. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für GLI3 Antikörper (AA 41-57) (ABIN303380)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reaktivität: Human, Ratte, Huhn, Hund, Affe, Wachtel, Frosch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-GLI3 Antikörper

Bindungsspezifität: AA 41-57

Spezifität: This antibody recognises GLI-Kruppel Family Member GLI3.

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, Chicken, Canine (Dog), Frog, Monkey, Quail and Rat.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic Peptide -KLH conjugated corresponding to Amino acids 41-57 of Human Gli-3 protein.

Anwendungsinformationen

Applikationshinweise: ELISA (1/6000-1/30000). Western Blot (1/500-1/2000). Immunohistochemistry on Paraffin Sections (1.25 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Details zu GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Andere Bezeichnung: GLI3

Hintergrund: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.Synonyms: GLI-3, Zinc finger protein GLI3

Gen-ID: 2737

NCBI Accession: NP_000159

UniProt: P10071

Pathways: Hedgehog Signalweg