CD79a Molecule, Immunoglobulin-Associated alpha (CD79A) Antikörper

Antigen: CD79a Molecule, Immunoglobulin-Associated alpha (CD79A)

Synonyme: IGA, MB-1, CD79A, IG-alpha

Klonalität: Monoklonal (HM57)

Wirt: Maus

Reaktivität: Human, Schwein, Maus, Ratte (Rattus), Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Opossum (Didelphimorphia), Huhn

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN302099

Produktbeschreibung

Weitere Bezeichnung: CD79a

Immunogen: Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: HM57

Beschreibung: CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They bothare transmembrane proteins with extended cytoplasmic domains containingimmunoreceptor tyrosine activation motives (ITAMs), and together with cell surfaceimmunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are thefirst components of BCR that are expressed developmentally. They appear on pro-B cells inassociation with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-Bcells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin andlater with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a ispresent as an intracellular component. CD79a/b complex interacts with Src-family tyrosinekinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites fordownstream signaling.

Spezifität: The antibody HM57 interacts with CD79a (Ig alpha), a 40-45 kDa subunit of B cellantigen-specific receptor (BCR) and its early developmental forms.HLDA V, WS Code BC cB018HLDA VI, WS Code BP 193HLDA VI, WS Code BP 89HLDA VI, WS Code B B103HLDA VI, WS Code B CD79.4

Anwendungen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Intracellular staining of CD79a with anti-CD79a (HM57) PE (gated on leukemic blast cells) in a patient with childhood B-precursor ALL.

Publikationen

Publikationen: Faldyna, Samankova, Leva et al.: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, 2007 (PubMed).