Caveolin-1 Antikörper (AA 73-84)

Produktnummer ABIN294089

Der Ziege Polyklonal anti-Caveolin-1 Antikörper wird verwendet zum Nachweis von Caveolin-1 in Proben von Human und Affe. Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für Caveolin-1 Antikörper (AA 73-84) (ABIN294089)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Caveolin-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Caveolin-1 Antikörper

Bindungsspezifität: AA 73-84

Spezifität: Human CAV1 / Caveolin. No cross-reactivity expected to Caveolin 2 and Caveolin 3

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset (100%) Orangutan, Guinea pig (92%) Mouse, Rat, Dog, Bat, Hamster, Rabbit, Horse (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DELSEKQVYDAH from an internal region of human CAV1 / Caveolin (NP_001744.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset (100%), Orangutan, Guinea pig (92%), Mouse, Rat, Dog, Bat, Hamster, Rabbit, Horse (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Andere Bezeichnung: CAV1 / Caveolin 1

Hintergrund: Name/Gene ID: CAV1

Synonyms: CAV1, Caveolin-1, CGL3, CAV, MSTP085, VIP21, BSCL3

Gen-ID: 857

NCBI Accession: NP_001744

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals