Retinoblastoma 1 (RB1) (pSer780) Antikörper

Antigen: Retinoblastoma 1 (RB1)

Synonyme: RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB

Epitope: pSer780

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN273453

Produktbeschreibung

Weitere Bezeichnung: Rb (Phospho-Ser780)

Swiss-Prot: P06400

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 780 (T-L-S P -P-I).

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Spezifität: Rb (phospho-Ser780) antibody detects endogenous levels of Rb only when phosphorylated at serine 780.

Anwendungen

Applikationshinweise: WB: 1:500~1:1000 IHC: 1:50~1:100

Konzentration: 100µg/100µl

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Lagerung: Store at -20° C /1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Rb (phospho-Ser780) antibody (E011132) .

Western blot analysis of extract from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (Ab-780) antibody (E021110, Lane 1 and 2) and Rb (phospho-Ser780) antibody (E011132, Lane 3 and 4).