3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) Antikörper

Antigen: 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1)

Synonyme: PDK1, PRO0461, MGC20087, MGC35290, Pdk1, PDPK1, pdpk1, MGC82080, B29, IGB, Igb, Igbeta, Ig-beta, MGC108607, CD79B, LOC100304995

Epitope: pSer241

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN273224

Produktbeschreibung

Weitere Bezeichnung: PDK1 (Phospho-Ser241)

Swiss-Prot: O15530

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-S P -F-V).

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Spezifität: PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.

Anwendungen

Applikationshinweise: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200

Konzentration: 50µg/50µl

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Lagerung: Store at -20° C /1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 (phospho-Ser241) antibody (E011005).

Western blot analysis of extracts from MDA-MB-435 cells using PDK1 (phospho-Ser241) antibody (E011005).