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Antikörper
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anti-phosphodiesterase 4D, CAMP-Specific (PDE4D) Antikörper
phosphodiesterase 4D, CAMP-Specific (PDE4D) Antikörper
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| Synonyme |
DPDE3, STRK1, HSPDE4D, PDE4DN2, FLJ97311, DKFZp686M11213 |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Enzyme Immunoassay (EIA), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Immunfluoreszenz (IF) (23), Western Blot (WB) (22), Durchflusszytometrie (FACS) (19), Immunpräzipitation (IP) (9), Enzyme Immunoassay (EIA) (8), ELISA (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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| Produktnummer |
ABIN272057 |
| Menge |
100 µg (1.0 mg/ml) (Varianten) |
| Preis |
320,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: PDE4D, DPDE3, cAMP-specific 3', 5'-cyclic phosphodiesterase 4D, EC=3.1.4.17, PDE43
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Weitere Bezeichnung
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PDE4D
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Swiss-Prot
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Q08499
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Kreuzreaktivität
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Human, Maus, Ratte (Rattus)
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Beschreibung
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Phosphodiesterases (PDE) hydrolyze cAMP to 5'AMP and thus play a critical role in theregulation of intracellular cAMP. Division of the PDE superfamily by sequence homologyand enzymatic properties yields 11 PDE families. A unique upstream conserved region(UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes,and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoformscontain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCRdomains are necessary for dimerization of PDE4 isoforms. The human PDE4D gene maps tochromosome 5q12. The splice variants include isoforms PDE4D1-6.
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Spezifität
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PDE4D antibody detects endogenous levels of PDE4D protein. Species: Human, Mouse, Rat. Others not tested.
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Applikationshinweise
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ELISA: 1: 10000~1: 20000. WB: 1: 500~1: 1000. IHC: 1: 50~1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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1.0 mg/ml
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Reinigung
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Aff - Purified
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
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Lagerung
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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Signaltransduktion, Enzyme, Metabolismus
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "phosphodiesterase 4D, CAMP-Specific (PDE4D)", Typ "Antikörper" finden
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Wirte
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Kaninchen (32), Ziege (8), Maus (1)
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Reaktivitäten
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Human (35), Maus (27), Ratte (Rattus) (27), Hund (2), Pferd (2), Huhn (1), Rind (Kuh) (1), Schwein (1), Schaf (1)
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Applikationen
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Immunfluoreszenz (IF) (23), Western Blot (WB) (22), Durchflusszytometrie (FACS) (19), Immunpräzipitation (IP) (9), Enzyme Immunoassay (EIA) (8), ELISA (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (1), pSer190 (1)
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