PLAT Antikörper

Produktnummer ABIN264767

Der Kaninchen Polyklonal anti-PLAT Antikörper wird verwendet zum Nachweis von PLAT in Proben von Human. Er wurde validiert für WB, IHC (fro) und EIA.

Kurzübersicht für PLAT Antikörper (ABIN264767)

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PLAT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-PLAT Antikörper

Spezifität: This antibody recognizes tPA.

Aufreinigung: Protein A Chromatography

Immunogen: Recombinant human tPA

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10,000 - 1: 100,000. Western blot: 1: 1,000 - 1: 10,000. Immunohistochemistry: 1: 100 - 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 4.5 mg/mL (OD280nm, E0.1% = 1.36)

Buffer: 0.05 M Sodium Phosphate, pH 6.6 containing 0.5 M Sodium Chloride and 1 mM EDTA

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -80 °C

Details zu PLAT

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Andere Bezeichnung: T-PA / PLAT

Hintergrund: TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, cell migration and many other physiopathological events. TPA binds to fibrin with high affinity, this leads to an increase in the catalytic efficiency of the enzyme of 100- to 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low density lipoprotein receptor related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. Binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.Synonyms: Alteplase, Reteplase, Tissue-type plasminogen activator, tPA

Gen-ID: 5327

NCBI Accession: NP_000921

UniProt: P00750

Pathways: Autophagie, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interaktom