GFP Antikörper

Produktnummer ABIN243767

Der Kaninchen Polyklonal anti-GFP Antikörper wird verwendet zum Nachweis von GFP in Proben von Aequorea victoria. Er wurde validiert für WB, IHC, IF, IP, ICC und IHC (p).

Kurzübersicht für GFP Antikörper (ABIN243767)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GFP Antikörper

Spezifität: Recognizes Green Fluorescent Protein (GFP). Species cross-reactivity: all variants of Aequorea victoria GFP, S65T-GFP, RS-GFP, YFP and EGFP. Detects 5 ng of recombinant GFP (ECL) with 1 minute exposure or less.

Aufreinigung: Affinity purified

Immunogen: Highly purified recombinant GFP made in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:200 - 1:1000), IF, IHC, IHC-P (1:2500 - 1:10000), IP, WB (1:2500 - 1:10000)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunogold Electron Microscopy. Western Blot: 1:2500-1:10000 in 1X BLOTTO or 3 % BSA in PBS. Detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins. Immunocytochemistry: 1:200-1:1000 in 4 % normal serum. Very good signal on recombinant YES-GFP chimeras expressed in COS cells. Immunohistochemistry: 1:200 with HRP detection systems. Has been shown to be useful on mouse sections fixed with formalin. Immunohistochemistry: 1:200 for paraffin sections. Requires heat mediated antigen retrieval via the microwave method before IHC staining (13 min in 10 mM citrate buffer  pH 6 followed by rapid cooling on ice). Immunoprecipitation: 1 μL per 10cm tissue culture dish of transfected cells. Use 10 μL protein A agarose CL4B to precipitate immune complex. Immunogold Electron Microscopy: 1:100.

Kommentare:

Target Species of Antibody: Aequorea victoria

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212