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Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D) Antikörper

Antigen

Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D)
Synonyme SHIP, SHIP1, hp51CN, SIP-145, MGC104855, MGC142140, MGC142142, INPP5D, MGC157261, inpp5d, ship, ship1, MGC68993
Klonalität Monoklonal (SHIP-02)
Wirt
Reaktivität
Alternativen

Human
Applikation
Alternativen Enzyme Immunoassay (EIA), Western Blot (WB)
Produktnummer ABIN238500
Menge 0.1 mg  (1.0 mg/ml)
Preis 200,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 7 bis 10 Werktagen

Produktbeschreibung
Anwendungen Alternativen Sekundärantikörper zurück nach oben

Produktmerkmale Synonyms: SHIP, SHIP1, SHIP-1, p150Ship, SIP-145, Phosphatidylinositol-3, 4, 5-trisphosphate5-phosphatase 1, SH2 domain-containing inositol-5'-phosphatase 1, Inositolpolyphosphate-5-phosphatase of 145 kDa
Weitere Bezeichnung INPP5D
Gen-ID 3635
Swiss-Prot Q92835
Immunogen Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Kreuzreaktivität Human
Isotyp IgG2a  (Passende Sekundärantikörper)
Klon SHIP-02
Beschreibung SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase thatregulates cell responses in lymphocytes and myeloid cells by hydrolyzing the secondmessenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitoryand activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine andgrowth factor receptors, and supresses PI3K-dependent signaling, down-regulates cellmigration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as ascaffold for the recruitment of other proteins to the plasma membrane.
Spezifität The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confinedto hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weightsof 110, 125, 130, 135 and 145 kDa. Species: Human.

Anwendungen
Alternativen Sekundärantikörper zurück nach oben

Applikationshinweise Western Blotting (Reducing conditions)Positive control: RAMOS human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Konzentration 1.0 mg/ml
Reinigung Aff - Purified
Buffer PBS, pH 7.4 with 15 mM sodium azide as preservative.
Lagerung Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen
Anwendungen Sekundärantikörper zurück nach oben

Alternativen zu Antigen "Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D)", Typ "Antikörper" finden
Wirte Kaninchen (45), Maus (6)
Reaktivitäten Human (49), Maus (29), Ratte (Rattus) (27), Rind (Kuh) (2), Schwein (2)
Applikationen Durchflusszytometrie (FACS) (25), Western Blot (WB) (24), Immunfluoreszenz (IF) (18), Immunhistochemie (Paraffinschnitte) (IHC (p)) (10), Enzyme Immunoassay (EIA) (9), ELISA (8), Immunpräzipitation (IP) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (IHC) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
Konjugate Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitope C-Term (5), Center (2), N-Term (2), pTyr1021 (1)