HLA Class II DR beta 1 (HLA-DRB1) Antikörper

Antigen: HLA Class II DR beta 1 (HLA-DRB1)

Synonyme: SS1, DRB1, DRw10, HLA-DRB, FLJ75017, FLJ76359, HLA-DR1B, HLA-DRB1*, HLA-DR3B, HLA-DR52, MGC117330, MGC108235

Klonalität: Monoklonal (MEM-267)

Wirt: Maus

Reaktivität: Human

Applikation: Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Western Blot (WB)

Produktnummer: ABIN238469

Produktbeschreibung

Produktmerkmale: Synonyms: MHC class II antigen DRB1, DR1, DR-1, HLA class II histocompatibility antigen, DRB1-1 betachain

Weitere Bezeichnung: HLA class II DR beta 1 / HLA-DRB1

Swiss-Prot: P04229

Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Kreuzreaktivität: Human

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: MEM-267

Beschreibung: HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is aheterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in themembrane. It plays a central role in the immune system by presenting peptides derivedfrom extracellular proteins. MHC Class II molecules are expressed in antigen presentingcells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the betachain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms isroutinely done for bone marrow and kidney transplantation.

Spezifität: The antibody MEM-267 specifically binds to the empty but not peptide-loaded form ofHLA-DR1. DR is the isotypes of human MHC Class II molecules expressed onantigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages). Species: Human. Others not tested.

Anwendungen

Applikationshinweise: Western Blotting. Flow Cytometry: The antibody MEM-267 stains immature dendritic cells that express emptycell surface MHC molecules, but not cells that express predominantly peptide loadedforms. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Aff - Purified

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar