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HLA-DRB1 Antikörper

HLA-DRB1 Reaktivität: Human, Affe WB, FACS, IF, IHC, ISt, StM Wirt: Maus Monoclonal LN-3 unconjugated
Produktnummer ABIN6939640
  • Target Alle HLA-DRB1 Antikörper anzeigen
    HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))
    Reaktivität
    • 95
    • 30
    • 11
    • 11
    • 11
    • 11
    • 10
    • 8
    Human, Affe
    Wirt
    • 54
    • 42
    Maus
    Klonalität
    • 53
    • 43
    Monoklonal
    Konjugat
    • 41
    • 9
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser HLA-DRB1 Antikörper ist unkonjugiert
    Applikation
    • 61
    • 45
    • 38
    • 25
    • 18
    • 17
    • 13
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
    Spezifität
    This MAb reacts with a 28 kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
    Keine Kreuzreaktivität
    Maus
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    Activated human peripheral blood mononuclear cells
    Klon
    LN-3
    Isotyp
    IgG2b kappa
    Top Product
    Discover our top product HLA-DRB1 Primärantikörper
  • Applikationshinweise

    Positive Control: Raji, Ramos, Daudi or HuT78 cells. Spleen, Tonsil or lymph node.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Haltbarkeit
    24 months
  • Target
    HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))
    Andere Bezeichnung
    HLA-DRB1 (HLA-DRB1 Produkte)
    Synonyme
    MGC108235 antikoerper, DRB1 antikoerper, DRw10 antikoerper, HLA-DR1B antikoerper, HLA-DRB antikoerper, SS1 antikoerper, major histocompatibility complex, class II, DR beta 1 antikoerper, hla-drb1 antikoerper, HLA-DRB1 antikoerper
    Molekulargewicht
    28kDa (beta chain)
    Gen-ID
    3123
    UniProt
    P01911
    Pathways
    T-Zell Rezeptor Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints
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