STIM1 / GOK Antikörper

Antigen: STIM1 / GOK

Klonalität: Monoklonal (CDN3H4)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN238397

Produktbeschreibung

Produktmerkmale: Synonyms: Stromal interaction molecule 1

Swiss-Prot: Q13586

Immunogen: Synthetized peptide (C-terminal cytoplasmic part of STIM1)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: CDN3H4

Beschreibung: STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calciumdepletion within the endoplasmic reticulum and transduces the signal to Orai1, thepresumptive CRAC channel at the plasma membrane. Following decrease of luminalcalcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry ofextracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1interaction has not been elucidated yet. Many questions also remain to be solved aroundSTIM1 functional distribution. It turns out that STIM1 associates with growing ends ofmicrotubules and is involved in endoplasmic reticulum tubule extension.

Spezifität: The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential andconserved regulator of store-operated Ca2+ channel function.

Anwendungen

Applikationshinweise: Immunoprecipitation. Western Blotting (reducing and non-reducing conditions): Recommended dilution: 1 μg/mlPositive control: RBL rat basophilic leukemia cell lineSample preparation: Resuspend approx.50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing/reducing Laemmli SDS-PAGE sample buffer. Immunocytochemistry: Staining technique: methanol-aceton fixation. Positive control: HeLa human cervix carcinoma cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mlOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Aff - Purified

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar