HLA Class I alpha G (HLAG) Antikörper

Antigen: HLA Class I alpha G (HLAG)

Klonalität: Monoklonal (5A6G7)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Fangantikörper), Durchflusszytometrie (FACS), Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN238372

Produktbeschreibung

Produktmerkmale: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Weitere Bezeichnung: HLA class I alpha G / HLA-G

Swiss-Prot: P17693

Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupledto ovalbumin.

Kreuzreaktivität: Human

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 5A6G7

Beschreibung: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of aheavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternatesplicing provides both soluble and membrane bound forms of HLA G, with two solubleisoforms and four membrane bound isoforms. HLA G may be part of the fetal protectionsystem and is expressed on fetal derived placental cells.

Spezifität: The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded byshedding from membrane-bound HLA-G forms that do not contain the intron 4-encodedepitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced

Anwendungen

Applikationshinweise: Western Blotting (Reducing Conditions): 12% AA SDS-PAGE. Positive Control: JEG-3 cell lysate. Flow Cytometry. Immunohistochemistry on Paraffin and Frozen Sections: 10-20 μg/ml. Positive tissue: placenta tissue. Immunocytochemistry. ELISA: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISAfor analysis of soluble HLA-G in combination with antibody ABIN238525. Positive Control: HeLa/HLA-G5 transfectants cell lysate. HeLa/HLA-G5 cell supernatant. Negative Control: HeLa cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.4 with 15 mM Sodium Azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar