HLA Class I alpha G (HLAG) Antikörper

Antigen: HLA Class I alpha G (HLAG)

Klonalität: Monoklonal (2A12)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN238366

Produktbeschreibung

Produktmerkmale: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Weitere Bezeichnung: HLA class I alpha G / HLA-G

Swiss-Prot: P17693

Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupledto ovalbumin.

Kreuzreaktivität: Human

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 2A12

Beschreibung: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of aheavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternatesplicing provides both soluble and membrane bound forms of HLA G, with two solubleisoforms and four membrane bound isoforms. HLA G may be part of the fetal protectionsystem and is expressed on fetal derived placental cells.

Spezifität: The antibody 2A12 allows to discriminate between soluble HLA-G protein yielded byshedding from membrane-bound HLA-G forms that do not contain the intron 4-encodedepitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively splicedmRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: Flow Cytometry: excellent for intracellular stainingWestern Blotting. Immunohistochemistry (paraffin and frozen sections). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar