Epidermal Growth Factor Receptor (EGFR) (prylated At Tyrosine 845) Antikörper

Antigen: Epidermal Growth Factor Receptor (EGFR)

Synonyme: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Epitope: prylated At Tyrosine 845

Klonalität: Monoklonal (12A3)

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN235076

Produktbeschreibung

Weitere Bezeichnung: Human Epidermal Growth Factor Receptor (EGFR), phosphorylated at Tyrosine 845

Immunogen: Phosphopeptide conjugate Format: Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature.

Format: Purified, Lyophilized

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 12A3

Beschreibung: Cell lysate of 2 x 106 EGF treated HepG2 cells.

Spezifität: Binding of EGF leads to autophosphorylation of the EGF Receptor at 5 tyrosine residues. In addition, EGF Receptor is phosphorylated by members of the src kinase family at different tyrosine residues, phosphotyrosine 845 in the activation loop being the major site (tyrosine 845 is not an autophosphorylation site). EGFR acquires its full kinase activity only upon autophosphorylation and phosphorylation at tyrosine 845. Clone 12A3 allows the detection of EGFR activation after interaction with src kinases. Specifically recognizes EGFR phosphorylated at Tyrosine 845. The antibody does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. Reacts with Human, mouse and rat.

Synonyme: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Anwendungen

Applikationshinweise: Immunoblotting: 0.1ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (20,000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5?g/ml K67123M for 1 hour at RT and developed by ECL (exp. time: 30 sec). ELISA: 0.1ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Storage: Store lyophilized product at -20?C. After reconstitution, aliquot and store at -80?C. Thawed aliquots may be stored at 2?8?C for up to 3 months. Warning:

Konzentration: 100ug/ml (prior to lyophilization)

Reinigung: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: Lyophilized from PBS, PEG and Sucrose. Preservative: 0.09% Sodium azide

Lagerung: Store lyophilized product at -20°C. After reconstitution, aliquot and store at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Warning:Reconstitute with 1ml water for 15 minutes at room temperature. Purification:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (20,000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5?g/ml ABIN235076 for 1 hour at RT and developed by ECL (exp. time: 30 sec).