GLI3 Antikörper (AA 41-57)

Produktnummer ABIN214604

Der Kaninchen Polyklonal anti-GLI3 Antikörper wird verwendet zum Nachweis von GLI3 in Proben von Human, Huhn, Hund, Xenopus laevis, Wachtel und Squirrel Monkey. Er wurde validiert für WB, IHC, IF, ELISA, ICC und IHC (p).

Kurzübersicht für GLI3 Antikörper (AA 41-57) (ABIN214604)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reaktivität: Human, Huhn, Hund, Xenopus laevis, Wachtel, Squirrel Monkey

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GLI3 Antikörper

Bindungsspezifität: AA 41-57

Spezifität: Amino acids 41-57 of human Gli-3 protein.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human GLI3.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:6000 - 1:30000), ICC, IF, IHC, IHC-P (1.25 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1.25 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Andere Bezeichnung: GLI3

Hintergrund: Name/Gene ID: GLI3

Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3

Gen-ID: 2737

UniProt: P10071

Pathways: Hedgehog Signalweg