Heat Shock 27kDa Protein 1 (HSPB1) Antikörper

Antigen: Heat Shock 27kDa Protein 1 (HSPB1)

Synonyme: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN197345

Produktbeschreibung

Produktmerkmale: Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein

Weitere Bezeichnung: HSPB1 / HSP27

Swiss-Prot: P04792

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived fromhuman HSP27 around the phosphorylation site of serine 15 (G-P-Sp-W-D).

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Beschreibung: Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of severalsmall heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis isinduced by elevated temperature, as well as estrogen in hormone responsive cells. Thisprotein is involved in stress resistance and actin organization. Interestingly, human HSP27also shares greater than 50% homology with low molecular weight Drosophila HSP's andmammalian a-crystalline lens protein. Because of the estrogen responsive nature ofHsp27, this protein has been studied extensively in human estrogen responsive tissuessuch as cervix, endometrium and breast tissue. This work has led to the suggestion thatHsp27 may be a useful marker in classifying various hormone sensitive tumors.

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. HSP27 antibody AP02671PU detects endogenous levels of total HSP27 protein. Species: Human. Others not tested.

Synonyme: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259

Anwendungen

Applikationshinweise: Western blot (1: 500-1: 1000)Immunohistochemistry (1: 50-1: 100)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Aff - Purified

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azideand 50% Glycerol.

Lagerung: Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar