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Antikörper
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anti-Pyruvate Dehydrogenase Kinase, Isoenzyme 1 (PDK1) (pSer241) Antikörper
Pyruvate Dehydrogenase Kinase, Isoenzyme 1 (PDK1) (pSer241) Antikörper
| Antigen |
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| Synonyme |
MGC108625, B830012B01, D530020C15Rik, PDK1 |
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Epitope
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (114), Maus (37), Ratte (Rattus) (37), Rind (Kuh) (4), Hund (4), Huhn (3), Schwein (3), Pferd (2), Affe (2), Kaninchen (2), Schaf (2)
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Applikation
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 Alternativen Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB), Immunfluoreszenz (IF)
Western Blot (WB) (87), Enzyme Immunoassay (EIA) (29), Immunfluoreszenz (IF) (28), Immunhistochemie (Paraffinschnitte) (IHC (p)) (22), Durchflusszytometrie (FACS) (19), ELISA (15), Immunhistochemie (IHC) (11), Immunpräzipitation (IP) (8), Dot Blot (Dot) (6), ELISA (Detektionsantikörper) (3), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (3), Immunzytochemie (ICC) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN196617 |
| Menge |
50 µg (1.0 mg/ml) |
| Preis |
220,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: PDK-1, PDK1, 3-phosphoinositide-dependent proteinkinase 1, hPDK1, PDPK1
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Weitere Bezeichnung
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PDPK1
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Swiss-Prot
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O15530
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from humanPDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).
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Kreuzreaktivität
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Human, Maus, Ratte (Rattus)
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Spezifität
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site. PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only whenphosphorylated at serine 241. Species: Human, Mouse and Rat. Others not tested.
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Applikationshinweise
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Suitable for use in Western blot (1: 500~1: 1000) and Immunohistochemistry (1: 50~1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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1.0 mg/ml
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Reinigung
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Aff - Purified
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Buffer
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Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl,0.02% Sodium Azide and 50% glycerol.
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Lagerung
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Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Pyruvate Dehydrogenase Kinase, Isoenzyme 1 (PDK1)", Typ "Antikörper" finden
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Wirte
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Kaninchen (97), Maus (14), Ziege (6)
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Reaktivitäten
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Human (114), Maus (37), Ratte (Rattus) (37), Rind (Kuh) (4), Hund (4), Huhn (3), Schwein (3), Pferd (2), Affe (2), Kaninchen (2), Schaf (2)
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Applikationen
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Western Blot (WB) (87), Enzyme Immunoassay (EIA) (29), Immunfluoreszenz (IF) (28), Immunhistochemie (Paraffinschnitte) (IHC (p)) (22), Durchflusszytometrie (FACS) (19), ELISA (15), Immunhistochemie (IHC) (11), Immunpräzipitation (IP) (8), Dot Blot (Dot) (6), ELISA (Detektionsantikörper) (3), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (3), Immunzytochemie (ICC) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Biotin (3), HRP (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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N-Term (2), pSer241 (1), pSer393 (1)
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