CD18 Antikörper (PE)

Antigen: CD18

Klonalität: Monoklonal (MEM-148)

Wirt: Maus

Reaktivität: Human

Konjugat: PE

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN192259

Produktbeschreibung

Immunogen: Peripheral blood mononuclear cells

Isotyp: IgG1

Klon: MEM-148

Beschreibung: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2(CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18).In most cases, the response mediated by the integrin is a composite of the functions of itsindividual subunits. These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severereduction of CD18 expression leads to the development of a psoriasiform skin disease.CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient tomediate leukotoxin-mediated cytolysis.

Spezifität: The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible inintact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa typeI transmembrane protein expressed on all leukocytes.HLDA VI, WS Code AS A052 This antibody does not react with: Porcine .

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with R-Phycoerythrin (PE)

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).