PAPPA Antikörper

Produktnummer ABIN191313

Zitiert in 1 Publikation. Der Maus Monoklonal anti-PAPPA Antikörper (Klon 5H9) (ABIN191313) detektiert spezifisch PAPPA in WB, ELISA, IP, ELISA (Detection) und ELISA (Capture). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PAPPA Antikörper (ABIN191313)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAPPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), ELISA (Detection), ELISA (Capture)

Klon: 5H9

Produktdetails anti-PAPPA Antikörper

Spezifität: Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and pro-MBP subunits). MAbs 11E4 and 5H9 are specific to proMBP subunit. IgG for MAb 11E4 IgG2a for MAbs 4G11, 10A5, 3C8, 10H9, 18A10 and 7A6 IgG2b for MAbs 5H9, 10E1 and 10E2

Kreuzreaktivität: Human

Aufreinigung: Purified

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays and immunoprecipitation. Recommended pairs for the detection of dPAPP-A antigen in blood of AMI patients and patients with unstable angina (coating - conjugate): 4G11 – 3C8 4G11 – 10H9 10E1 – 7A6 Best pairs for the detection of htPAPP-A antigen in pregnancy serum (coating - conjugate): 4G11 – 3C8 4G11 – 10H9 10E2 – 10E1 10E2 – 5H9 5H9 – 10E2 10E1 – 7A6 PAPP-A immunodetection in Western blotting. MAb 18A10 recognizes PAPP-A subunit in reducing and non-reducing conditions after antigen transfer onto nitrocellulose membrane after SDS-gel electrophoresis. MAbs 4G11, 10A5, 18A10 and 10E1 recognize htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-PAPPA Antikörper (ABIN191313)

Qin, Christiansen, Pettersson: "Point-of-care time-resolved immunofluorometric assay for human pregnancy-associated plasma protein A: use in first-trimester screening for Down syndrome." in: Clinical chemistry, Vol. 48, Issue 3, pp. 473-83, (2002) (PubMed).

Details zu PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Andere Bezeichnung: Pappalysin-1 / PAPPA