Protein Inhibitor of Activated STAT, 2 (PIAS2) Antikörper

Antigen: Protein Inhibitor of Activated STAT, 2 (PIAS2)

Synonyme: miz, MIZ1, SIZ2, PIASX, ZMIZ4, MGC102682, PIASX-BETA, PIASX-ALPHA, Miz1, MGC83751, PIAS2, wu:fi05f09

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN190909

Produktbeschreibung

Weitere Bezeichnung: PIAS2

Gen-ID: 9063, 17344 (mouse)

Immunogen: KEAMKVSSQPCTKIE

Format: Affinity-purified

Beschreibung: All names and symbols: PIAS2, protein inhibitor of activated STAT, 2 , MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, miz , Msx-interacting-zinc finger, protein inhibitor of activated STAT X, ARIP3. Official Symbol: PIAS2. RefSeq number(s): NP_775298.1, NP_004662.2

Spezifität: This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4. Human, Mouse, Rat, Dog

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.2kDa according to NP_004662.2. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Proteolyse / Ubiquitin, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: van den Akker, Ano, Shih et al.: "FLI-1 functionally interacts with PIASxalpha, a member of the PIAS E3 SUMO ligase family." in: The Journal of biological chemistry, Vol. 280, Issue 45, pp. 38035-46, 2005 (PubMed).