Isocitrate Dehydrogenase (IDH) Antikörper

Antigen: Isocitrate Dehydrogenase (IDH)

Synonyme: CT22171, IDH, IDH-NADP, l(3)L3852, DmelCG7176, CG7176, F20D21.16, F20D21_16, ICDH, ISOCITRATE DEHYDROGENASE, icd, F6P23.14, F6P23_14, IDH2, ISOCITRATE DEHYDROGENASE SUBUNIT 2, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Erbse (Pisum sativum), Kartoffel (Solanum tuberosum), Maize/Corn (Zea mays)

Applikation: Western Blot (WB)

Produktnummer: ABIN190717

Produktbeschreibung

Swiss-Prot: P93032

Immunogen: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plantsmitochondrial, NAD dependent isocitrate dehydrogenase subunits includingArabidopsis thaliana IDH-I Q8LFCO and IDH-II P93032

Format: Lyophilized

Beschreibung: Plant NADH dependent isocitrate dehydrogenase enzyme is located inmitochondrial matrix. This enzyme is classified as an oxidoreductase and itsfunction is to catalyze a reaction in the citric acid cycle, specifically the sequentialdehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. Itremoves hydrogens from its substrate, isocitrate. In addition to this process, itfunctions as a decarboxylase, removing a CO2 from the six-carbon substrate toform a five-carbon

Spezifität: Predicted reactivity: dicots including Brassica napus, Vitis vinifera, monocots including Oryza sativa,Zea mays. Not reactive in: Chlamydomonas reinhardtii.

Synonyme: CT22171, IDH, IDH-NADP, l(3)L3852, DmelCG7176, CG7176, F20D21.16, F20D21_16, ICDH, ISOCITRATE DEHYDROGENASE, icd, F6P23.14, F6P23_14, IDH2, ISOCITRATE DEHYDROGENASE SUBUNIT 2, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2

Anwendungen

Applikationshinweise: Recommended Dilution 1 : 5 000 with standard ECL (WB). Additional Information: Peptide used to elicit this antibody is not conserved in NADPH dependentanzymes, partially conserved across eukaryotic Idh subunits. Some conservationacross bacterial which contain the NAD-dependent form of Idh (as opposed to theNADP-dependent form).

Buffer: Affinity purified serum in PBS pH 7.4

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure description: 1: Total extract A. thaliana (20 g protein) 2: Fraction enriched with mitochondria A. thaliana 3: Pure mitochondria A. thaliana 4: Pure mitochondria P. satisum 5: Pure mitochondria S. tuberosum Description of experimental conditions: After SDS-PAGE gel electrophoresis samples have been transferred to nitrocellulose membrane. Blocking has been done in 5% milk powder in TBS followed by incubation with primary antibodies for 1 hour and 30 minutes in RT After incubation with secondary antibodies reaction has been developed using ECL reagent (GE Healthcare) * Band detected at ca. 90 kDa is suspected to be a dimmer of Idh, since this band is depleted upon peptide competition experiment.

Publikationen

Publikationen: Szal, Jastrz?bska, Kulka et al.: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, 2010 (PubMed).