Isocitrate Dehydrogenase Antikörper

Produktnummer ABIN5540830

Produktdetails anti-Isocitrate Dehydrogenase Antikörper

Target: Isocitrate Dehydrogenase (IDH)

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Isocitrate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunocytochemistry (ICC)

Spezifität: Isocitrate Dehydrogenase isolated and purified from Porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Species: Pig.

Aufreinigung: Solid Phase Affinity Chromatography

Immunogen: Isocitrate Dehydrogenase isolated and purified from Porcine heart. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunoprecipiation. Can be Used: • In precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). • To prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/30,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Andere Bezeichnung: isocitrate dehydrogenase,idh (IDH Produkte)

Synonyme: CG7176 antikoerper, CT22171 antikoerper, Dmel\\CG7176 antikoerper, IDH antikoerper, IDH-NADP antikoerper, Idh-NADP antikoerper, idh antikoerper, isocitrate dehydrogenase antikoerper, l(3)L3852 antikoerper, F20D21.16 antikoerper, F20D21_16 antikoerper, SMU.672 antikoerper, icd antikoerper, Isocitrate dehydrogenase antikoerper, isocitrate dehydrogenase antikoerper, isocitrate dehydrogenase, NADP-dependent antikoerper, Monomeric isocitrate dehydrogenase antikoerper, ankyrin repeat domain 26-like antikoerper, Idh antikoerper, ICDH antikoerper, idh antikoerper, icd antikoerper, SSO_RS10660 antikoerper, JK_RS08640 antikoerper, LOC618625 antikoerper

Hintergrund: Isocitrate dehydrogenase is an enzyme involved in the citric acid cycle. It is 416 amino acids long with a molecular weight of approximately 45 kDa. Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate. The human genome has 5 IDH genes coding for 3 IDH enzymes. The IDH1 and IDH2 require nicotinamide adeninedinucleotide phosphate (NADP) as co-substrate, whereas IDH3 require nicotinamide adenine dinucleotide (NAD). The IDH2 and 3 are localized in mitochondria and are actively involved in the citric acid cycle (TCA) for energy production in contrast, IDH1 is localized in cytoplasm and peroxisomes where it generates NADPH, reduced form of NADP for biosynthetic and other types of reaction. Since alfa KG and NADPH both are intermediately substrate for a number of cellular process, which allows the possibility of oncogenic or tumor suppressive activities of IDH1.

UniProt: P20304