MGMT (AA 8-221) Antikörper

Antigen: MGMT

Epitope: AA 8-221

Klonalität: Monoklonal (MT3-1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN187469

Produktbeschreibung

Weitere Bezeichnung: MGMT (8-221)

Immunogen: Purified recombinant human MGMT protein.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MT3-1

Beschreibung: Cancer chemotherapeutic alkylating agents (e.g. BCNU) act by inducing formation of lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-methylguanine-DNA methyltransfer-ase) transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug-resistant.

Spezifität: Species Reactivity: Human. Cross-reacts weakly with mouse, rat and hamster. Other species not tested. Molecular Weight: .25 kDa Cellular Location: Nuclear and cytoplasmic.Positive Control: Raji cells, Colon carcinoma.

Anwendungen

Applikationshinweise: Flow cytometry Immunohistochemistry: (formalin / paraffin) Use at a 1:20 - 1:40 dilution for 60 minutes at room temperature (RT). Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 - 20 minutes followed by cooling at RT for 20 minutes. Note: IHC requires the use of a high-efficiency detection system. Western blot: Use at 0.5-1 µg/mL for 2 hours at RT. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 500 µL of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.

Lagerung: Store at 4 °C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-MGMT (AA 8-221) antibody

anti-MGMT (AA 8-221) antibody (Image 2)

Publikationen

Publikationen: Reese, Roth, Gerson: "Bone marrow-derived cells exhibiting lung epithelial cell characteristics are enriched in vivo using methylguanine DNA methyltransferase-mediated drug resistance." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 3, pp. 675-81, 2008 (PubMed).

Rietschel, Wolchok, Krown et al.: "Phase II study of extended-dose temozolomide in patients with melanoma." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 14, pp. 2299-304, 2008 (PubMed).

Cui, Johnson, Bullock et al.: "Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway." in: Molecular pharmacology, Vol. 75, Issue 6, pp. 1356-63, 2009 (PubMed).