Duffy Blood Group, Chemokine Receptor (DARC) Antikörper

Antigen: Duffy Blood Group, Chemokine Receptor (DARC)

Synonyme: DARC, FY, Dfy, GPD, GpFy, CCBP1, CD234, WBCQ1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC), Durchflusszytometrie (FACS)

Produktnummer: ABIN185480

Produktbeschreibung

Weitere Bezeichnung: Duffy / FY / DARC

Gen-ID: 2532

Immunogen: HRAELSPSTENSSQLDFED-C

Format: Affinity-purified

Beschreibung: All names and symbols: FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, Duffy blood group, chemokine receptor, Duffy blood group antigen, Glycoprotein D. Official Symbol: DARC. RefSeq number(s): NP_002027.2

Spezifität: Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 35kDa band observed in human kidney and human liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn Lung showing endothelial staining. Recommended concentration, 3µg/ml. FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185480 (0.1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185480 (3µg/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Publikationen

Publikationen: Bruehl, Vielhauer, Weiss et al.: "Expression of DARC, CXCR3 and CCR5 in giant cell arteritis." in: Rheumatology (Oxford, England), Vol. 44, Issue 3, pp. 309-13, 2005 (PubMed).