Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) Antikörper

Antigen: Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1)

Synonyme: B23, NPM, MGC104254, Npm, NO38, MGC102162, MGC107291, B23NP, MGC108517, npm1, MGC53901, npm

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185372

Produktbeschreibung

Weitere Bezeichnung: Nucleophosmin / NPM1

Gen-ID: 4869

Immunogen: C-QEAIQDLWQWRKSL

Format: Affinity-purified

Beschreibung: All names and symbols: NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1. Official Symbol: NPM1. RefSeq number(s): NP_002511.1, NP_954654.1. Gene ontology terms: NF-kappaB binding, RNA binding, Tat protein binding, activation of NF-kappaB transcription factor, cell aging, centrosome, centrosome cycle, cytoplasm, intracellular protein transport, negative regulation of cell proliferation, nucleocytoplasmic transport, nucleolus, protein heterodimerization activity, protein homodimerization activity, response to stress, ribosome assembly, transcription coactivator activity, unfolded protein binding

Spezifität: This antibody is expected to recognise both report isoforms (NP_002511.1, NP_954654.1). Human, Mouse, Rat, Dog, Chimpanzee 294 aa, 295aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 37kDa band observed in Human HeLa lysates (calculated MW of 32.6kDa according to NP_002511.1 and 29.5kDa according to NP_954654.1). Recommended concentration: 0.1-0.3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185372 (0.1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Grisendi, Pandolfi: "NPM mutations in acute myelogenous leukemia." in: The New England journal of medicine, Vol. 352, Issue 3, pp. 291-2, 2005 (PubMed).

Zhang, Garcia-Gras, Wycuff et al.: "Identification of direct serum-response factor gene targets during Me2SO-induced P19 cardiac cell differentiation." in: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 19115-26, 2005 (PubMed).