POLR2B Antikörper (Middle Region)

Produktnummer ABIN182577

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-POLR2B Antikörper (ABIN182577) detektiert spezifisch POLR2B in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Rind (Kuh), Zebrafisch (Danio rerio), Meerschweinchen, Pferd und Kaninchen.

Kurzübersicht für POLR2B Antikörper (Middle Region) (ABIN182577)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Zebrafisch (Danio rerio), Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-POLR2B Antikörper

Bindungsspezifität: Middle Region

Sequenz: FFRSVFYRSY KEQESKKGFD QEEVFEKPTR ETCQGMRHAI YDKLDDDGLI

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against POLR2B. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR2B

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1174 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-POLR2B Antikörper (ABIN182577)

Lee, Skalnik: "Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes." in: Molecular and cellular biology, Vol. 28, Issue 2, pp. 609-18, (2008) (PubMed).

Details zu POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Andere Bezeichnung: POLR2B

Hintergrund: POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Alias Symbols: RPB2, POL2RB, hsRPB2, hRPB140
Protein Interaction Partner: ESR1, UBC, TCEB3, SRC, PSMB9, SUMO2, XRN2, WWOX, SUZ12, RNF2, NEDD4, ITCH, RABEP2, HECW2, FBXO6, CDK20, HDAC11, ILK, POLR2C, MED19, MED26, RECQL5, VCP, BARD1, MDC1, POLR3F, TUBB4B, TUBA4A, POLR2I, POLR2E, POLR2D, POLR2A, DCTN1, ATP6V1B2, MEN1, CDK7, CCNH,
Protein Size: 1174

Molekulargewicht: 134 kDa

Gen-ID: 5431

NCBI Accession: NM_000938, NP_000929

UniProt: P30876

Pathways: Regulatorische RNA Pathways, DNA Reparatur