VEGFA Antikörper

Produktnummer ABIN155189

Der Kaninchen Polyklonal anti-VEGFA Antikörper wird verwendet zum Nachweis von VEGFA in Proben von Human und Maus. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für VEGFA Antikörper (ABIN155189)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VEGFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-VEGFA Antikörper

Spezifität: The antibody recognizes VEGF-A under reducing and non-reducing conditions.

Kreuzreaktivität (Details): Species reactivity (tested):Human and Mouse.

Aufreinigung: Antigen Affinity Chromatography using recombinant Murine VEGF164 as matrix

Immunogen: Recombinant Murine VEGF164 Dimer

Anwendungsinformationen

Applikationshinweise: ELISA: To detect murine VEGF-A by direct ELISA a concentration of at least 0.5 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 0.5-1.0 ng/well of recombinant murine VEGF165 or other VEGF-A splice forms. Western Blot: To detect murine VEGF-A by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections (Pretreatment: 0.01 % Proteinase K, 15 min atRT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of > 0.5 mg/mL.

Buffer: PBS, pH 7.2 without preservatives and stabilizers

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE.

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGF-A

Hintergrund: VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gen-ID: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling