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CD3 (CD3E) Antikörper (Biotin)

Antigen

CD3 (CD3E)
Synonyme T3E, TCRE, FLJ18683
Klonalität Monoklonal (CT-3)
Wirt
Reaktivität
Alternativen

Huhn, Vogel
Konjugat
Alternativen Biotin
Applikation
Alternativen Durchflusszytometrie (FACS), Immunpräzipitation (IP), Blockierende Antikörper (Inhibition)
1 Publikation vorhanden
Produktnummer ABIN135506
Menge 0,5 mg
Preis 300,59 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 bis 7 Werktagen

anti-CD3 (CD3E) antibody (Biotin)

Produktbeschreibung
Anwendungen Publikationen Bilder Alternativen zurück nach oben

Weitere Bezeichnung CD3
Isotyp IgG1
Klon CT-3
Beschreibung Chicken CD3 CD3 is a member of the T cell receptor-associated CD3 complex. MAb CT-3 recognizes a complex of at least three polypeptides of Mr 20-, 19-, and 17-kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90-kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50- and 40-kDa.

Anwendungen
Publikationen Bilder Alternativen zurück nach oben

Applikationshinweise Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.5 ug/10^6 cells), Spectral RedTM (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Lagerung The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral RedTM (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Forschungsgebiet Immunologie, Adaptive Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen
Beschränkungen Nur für Forschungszwecke einsetzbar

Publikationen
Anwendungen Bilder Alternativen zurück nach oben

Publikationen Cheng, Zhu, Sun: "The immune enhancement of sodium lauryl sulfoacetate in chickens." in: Veterinary medicine international, Vol. 2010, pp. 485060, 2010 (PubMed).

Alternativen
Anwendungen Publikationen Bilder zurück nach oben

Alternativen zu Antigen "CD3 (CD3E)", Typ "Antikörper" finden
Wirte Maus (478), Ratte (85), Kaninchen (61), Hamster (54)
Reaktivitäten Human (438), Maus (153), Ratte (Rattus) (64), Schwein (34), Hund (30), Huhn (22), Affe (19), Rind (Kuh) (17), Primate (10), Katze (4), Vogel (3), Hamster (3), Pferd (3), Primaten (3), Rhesusaffen (2), Schimpanse (1), Kaninchen (1)
Applikationen Durchflusszytometrie (FACS) (562), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (143), Immunpräzipitation (IP) (122), Western Blot (WB) (59), Funktionale Studien (Func) (58), Immunhistochemie (Paraffinschnitte) (IHC (p)) (54), Immunfluoreszenz (IF) (47), Immunhistochemie (IHC) (27), ELISA (19), Blockierende Antikörper (Inhibition) (9), Enzyme Immunoassay (EIA) (9), Immunzytochemie (ICC) (8), Aktivierung (Act) (4), Radioimmunoassay (RIA) (3), Zytotoxizitätstest (CyTox) (2), Abreicherung (Depletion) (2), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
Konjugate FITC (106), PE (67), Biotin (66), APC (32), Alexa Fluor 488 (13), RPE (10), Alexa Fluor 647 (9), Alexa Fluor 700 (9), Cy5 (9), Pacific Blue (8), PerCP,Cy5.5 (8), PE,Cy5 (7), SPRD (7), APC,Cy7 (6), EFluor 450 (6), PE,Cy7 (6), Cy5,PE (4), PerCP (4), DY647 (3), APC-eFluor 780 (2), Cy7,PE (2), EFluor 605NC (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), RBITC (1), RPE,Cy7 (1)
Epitope AA 156-168 (4), AA 196-206 (1), C-Term (1)