HSP90AA1 Antikörper (AA 289-300)

Produktnummer ABIN129685

Hersteller Produkt- Nr.: 600-401-929

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch HSP90AA1 in WB, IHC und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Affe, Huhn und Drosophila melanogaster.

Kurzübersicht für HSP90AA1 Antikörper (AA 289-300) (ABIN129685)

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Reaktivität: Human, Maus, Ratte, Affe, Huhn, Drosophila melanogaster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSP90AA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-HSP90AA1 Antikörper

Bindungsspezifität: AA 289-300

Hersteller Produkt- Nr.: 600-401-929

Hersteller: Rockland

Verwendungszweck: HSP90 alpha Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against human Hsp90 protein.

Produktmerkmale: Synonyms: rabbit anti-Heat Shock Protein HSP 90-alpha antibody, HSP 86 antibody, Heat shock 86 kDa antibody, Renal carcinoma antigen NY REN 38 antibody, Heat shock 90 kDa protein 1 alpha antibody, Lipopolysaccharide-associated protein 2, LAP-2, HSP90A, HSPC1, HSPCA, HSP90AA1, HSP 90-α

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 289-300 of human Hsp90 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.24 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu HSP90AA1

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Andere Bezeichnung: HSP90AA1

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species.  Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha).  Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG).  The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out).  One possible mechanism may be differences in post-translational modification of tumor Hsp90.

Gen-ID: 3320

NCBI Accession: NP_001017963

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals