Apg7 Antikörper

Antigen: Apg7

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129600

Produktbeschreibung

Gen-ID: NP_006386

Swiss-Prot: Q9BWP2

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding aa 558-569 of Human Apg7 protein. Protein Sequence: Human Apg7, 703 aa, predicted MW 78.0 kDa 1 maaatgdpgl sklqfapfss aldvgfwhel tqkklneyrl deapkdikgy yyngdsaglp 61 arltlefsaf dmsaptparc cpaigtlynt ntlesfktad kkllleqaan eiwesiksgt 121 alenpvllnk fllltfadlk kyhfyywfcy palclpeslp liqgpvgldq rfslkqieal 181 ecaydnlcqt egvtalpyfl ikydenmvlv sllkhysdff qgqrtkitig vydpcnlaqy 241 pgwplrnflv laahrwsssf qsvevvcfrd rtmqgardva hsiifevklp emafspdcpk 301 avgweknqkg gmgprmvnls ecmdpkrlae ssvdlnlklm cwrlvptldl dkvvsvkcll 361 lgagtlgcnv artlmgwgvr hitfvdnaki sysnpvrqpl yefedclggg kpkalaaadr 421 lqkifpgvna rgfnmsipmp ghpvnfssvt leqarrdveq leqlieshdv vfllmdtres 481 rwlpaviaas krklvinaal gfdtfvvmrh glkkpkqqga gdlcpnhpva sadllgsslf 541 anipgyklgc yfcndvv apg dstrdrtld q qctvsrpgla viagalavel mvsvlqhpeg 601 gyaiasssdd rmnepptslg lvphqirgfl srfdnvlpvs lafdkctacs skvldqyere 661 gfnflakvfn sshsfledlt gltllhqetq aaeiwdmsdd eti

Format: Liquid (sterile filtered)

Beschreibung: Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 andSaccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal andvaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activatingenzymes.

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using theimmunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However,BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, andchicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D.melanogaster. Reactivity with Apg7 proteins from other sources is not known.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in sizecorresponding to human Apg7 by western blotting in the appropriate cell lysate or extract. Human Apg7 has beenreported to form a homodimer at approximately 160 kDa. Dilutions: ELISA 1:15,000 - 1:75,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 1.46 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Forschungsgebiet: Krebs, Autophagie, Metabolismus, Proteolyse / Ubiquitin, Apoptose/Nekrose

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Sarkar, Perlstein, Imarisio et al.: "Small molecules enhance autophagy and reduce toxicity in Huntington's disease models." in: Nature chemical biology, Vol. 3, Issue 6, pp. 331-8, 2007 (PubMed).