Checkpoint Kinase 2 (CHEK2) (pT68) Antikörper

Antigen: Checkpoint Kinase 2 (CHEK2)

Synonyme: CDS1, CHK2, LFS2, RAD53, HuCds1, PP1425

Epitope: pT68

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129576

Produktbeschreibung

Weitere Bezeichnung: CHK2 pT68

Swiss-Prot: O96017

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding aa 64-73 of Human CHK2. Protein Sequence: Human CHK2, 543 aa, predicted MW 60.9 kDa 1 msresdveaq qshgssacsq phgsvtqsqg sssqsqgiss sststmpnss qsshsssgtl 61 ssletvs t qe lysipedqep edqepeeptp apwarlwalq dgfanlecvn dnywfgrdks 121 ceycfdepll krtdkyrtys kkhfrifrev gpknsyiayi edhsgngtfv ntelvgkgkr 181 rplnnnseia lslsrnkvfv ffdltvddqs vypkalrdey imsktlgsga cgevklafer 241 ktckkvaiki iskrkfaigs areadpalnv eteieilkkl nhpciikikn ffdaedyyiv 301 lelmeggelf dkvvgnkrlk eatcklyfyq mllavqylhe ngiihrdlkp envllssqee 361 dclikitdfg hskilgetsl mrtlcgtpty lapevlvsvg tagynravdc wslgvilfic 421 lsgyppfseh rtqvslkdqi tsgkynfipe vwaevsekal dlvkkllvvd pkarftteea 481 lrhpwlqded mkrkfqdlls eenestalpq vlaqpstsrk rpregeaega ettkrpavca 541 avl

Format: Liquid (sterile filtered)

Beschreibung: CHK2 (also known as CHEK2, Protein kinase CHK2 isoform a, and checkpoint-like protein) is a serine/threonine-protein kinase involved in the control of cell cycle checkpointsand may also participate in transduction of the DNA damage andreplicational stress signals. CHK2 is the mammalian ortholog of thebudding yeast Rad53 and fission yeast Cds1 checkpoint kinases. Theamino-terminal domain of CHK2 contains a series of seven serine andthreonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68)followed by glutamine (SQ or TQ motif). These are known to be preferredsites for phosphorylation by ATM/ATR kinases. Indeed, after DNAdamage by ionizing radiation (IR), UV irradiation or hydroxyureatreatment, Thr68 and other sites in this region become phosphorylated byATM/ATR. The SQ/TQ cluster domain, therefore, seems to have aregulatory function. Phosphorylation at Thr68 is a prerequisite for thesubsequent activation step, which is attributable to autophosphorylation ofChk2 on residues Thr383 and Thr387 in the activation loop of the kinasedomain. CHK2 inhibits CDC25C phosphatase by phosphorylating it onSer-216, preventing the entry into mitosis. This kinase may have a role inmeiosis as well. Kinase activity is up regulated by autophosphorylationand the protein is rapidly phosphorylated in response to DNA damage andto replication block. CHK2 shows a nuclear localization and is highly expressed in testis, spleen, colon and peripheral blood leukocytes. Low levels ofexpression are found in other tissues. Defects in CHEK2 are associated with Li-Fraumeni syndrome (LFS), a highly penetrant familialcancer phenotype usually associated with inherited mutations in p53/TP53. Defects in CHEK2 are also found in some patients withprostate cancer (CaP) or osteosarcoma (OSRC). Substantial amino acid variants exist in some cancer tissues. Numerous truncatedsplice variants exist for this protein.

Spezifität: This affinity purified antibody is directed against the phosphorylated form of human CHK2 at thepT68 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum wasfirst purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then crossadsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human CHK2 pT68protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated humanCHK2 is minimal by ELISA. The antibody does not cross-react with Chk2 phosphorylated at other sites. A BLAST analysiswas used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogensequence. Cross reactivity with CHK2 protein from mouse and rat may occur as sequence homology varies by one aminoacid residues in this sequence (90% homology). Cross reactivity with CHK2 homologues from other sources has not beendetermined.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditionsfor reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 bywestern blotting in the appropriate cell lysate or extract. Less than 1% reactivity is observed against the non-phosphorylatedform of the immunizing peptide. This antibody is phospho specific for pT68 of CHK2.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Forschungsgebiet: Kinasen/Phosphatasen, Zellzyklus, Phospho-Antikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Checkpoint Kinase 2 (CHEK2) (pT68) antibody

Publikationen

Publikationen: Lu, Lou, Peng et al.: "Effect of caffeine on the ATR/Chk1 pathway in the epidermis of UVB-irradiated mice." in: Cancer research, Vol. 68, Issue 7, pp. 2523-9, 2008 (PubMed).