Polycomb Group Ring Finger 4 (BMI1) Antikörper

Antigen: Polycomb Group Ring Finger 4 (BMI1)

Synonyme: PCGF4, RNF51, MGC12685

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129562

Produktbeschreibung

Weitere Bezeichnung: Bmi1

Gen-ID: NP_005171

Swiss-Prot: P35226

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein. Protein Sequence: Human Bmi1, 326 aa, predicted MW 36.9 kDa 1 mhrttrikit elnphlmcvl cggyfidatt iieclhsfck tcivrylets kycpicdvqv 61 hktrpllnir sdktlqdivy klvpglfkne mkrrrdfyaa hpsadaangs nedrgevade 121 dkriitddei islsieffdq nrldrkvnkd kekskeevnd krylrcpaam tvmhlrkflr 181 skmdipntfq idvmyeeepl kdyytlmdia yiytwrrngp lplkyrvrpt ckrmkishqr 241 dgltnagele sdsgsdkans paggipstss clpspstpvq sphpqfphis stmngtsnsp 301 sgnhqssfan rprkssvngs satssg

Format: Liquid (sterile filtered)

Beschreibung: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.

Spezifität: This affinity purified antibody is directed against human Bmi1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Bmi1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Bmi1 by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 15,000 - 130,000 WESTERN BLOT 1500 - 13,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 0.79 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Krebs, Zellzyklus, Hämopoetische Vorläufer, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using antibodies-online's Affinity Purified anti-Bmi1 antibody shows detection of a band ~37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4