Microtubule Associated Protein 2 (MAP2) Antikörper

Antigen: Microtubule Associated Protein 2 (MAP2)

Synonyme: MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864

Klonalität: Monoklonal (MT-08)

Wirt: Maus

Reaktivität: Human, Schwein, Maus

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA, Radioimmunoassay (RIA), Immunzytochemie (ICC)

Produktnummer: ABIN125739

Produktbeschreibung

Weitere Bezeichnung: MAP2ab

Immunogen: Microtubule protein (bovine brain) enriched for kinesin

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MT-08

Beschreibung: MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift thereaction kinetics in addition of new subunits and microtubule growth) and participate indetermining the structure of different parts of vertebrate nerve cells.

Spezifität: The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain ofmolecule Microtubule Associated Protein 2ab (MAP2ab).

Anwendungen

Applikationshinweise: Immunoprecipitation - Positive material: Porcine brainWestern Blotting - Positive control: Porcine brainMicrotubule associated protein 2 (MAP2) - Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: brainImmunohistochemistry (frozen sections) - Positive tissue: Mouse brain. Immunocytochemistry: Positive control: human neuroblastoma SH-SY5YELISA - Positive control: Porcine brainMicrotubule associated protein 2 (MAP2)

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blotting

Publikationen

Publikationen: Dzijak, Yildirim, Kahle et al.: "Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner." in: PLoS ONE, Vol. 7, Issue 1, pp. e30529, 2012 (PubMed).