CD44 (CD44) Antikörper (PE)

Antigen: CD44 (CD44)

Synonyme: IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468

Klonalität: Monoklonal (MEM-85)

Wirt: Maus

Reaktivität: Human

Konjugat: PE

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN125694

Produktbeschreibung

Weitere Bezeichnung: CD44

Immunogen: Leukocytes of a patient suffering from LGL Type Leukaemia.

Isotyp: IgG2b

Klon: MEM-85

Beschreibung: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, whichserves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion,proliferation and migration, it is thus important for lymphocyte activation, recirculation andhoming, it can thus serve e.g. as a modulator of macrophage recruitment in response topathogen. Although CD44 functions are essential for physiological activities of normal cells,elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitatingtumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Spezifität: The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherinfamily) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymalcells, etc.), it is negative on platelets and hepatocytes.HLDA IV, WS Code NL 706HLDA VI, WS Code AS Ref.15

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, CD Antigene, Oberflächenrezeptoren der Immunzellen , Krebs, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).