Blood Group Lewis B Antikörper

Antigen: Blood Group Lewis B

Klonalität: Monoklonal (2-25LE)

Wirt: Maus

Reaktivität: Human

Applikation: Enzyme Immunoassay (EIA), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN120686

Produktbeschreibung

Weitere Bezeichnung: Blood Group Antigen Lewis b

Immunogen: Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 2-25LE

Spezifität: The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. It does not react with the blood group H type 2 antigens or with Lewis A, Sialyl Lewis A, Lewis X, Sialyl Lewis X, Lewis Y, and Lewis C.

Anwendungen

Applikationshinweise: Immunohistochemistry: 2-4 ug/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Use human colon CA for postive control. Western blot. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS, pH 7.4, with 15 mM sodium azide as preservative

Lagerung: Store at 2-8°C for 6 months. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. Shelf Life: one year from despatch.

Forschungsgebiet: Zell-/Gewebemarker, Blutgruppen-Antigene, Blutbestandteile

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Formalin-fixed, paraffin-embedded human colon stained with Lewis B Ab (Cat. SM3092) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells.